thiC [2019-02-19 19:13:53]

4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase, biosynthesis of the pyrimidine moiety of thiamine

Locus: BSU08790

Isoelectric point: 5.26

Molecular weight: 65.74 kDa

Protein length: 590 aa

Gene length: 1773 bp

Function: biosynthesis of thiamine

Product: 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase

Essential: no

Synonyms: thiA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

Gene

Coordinates: 955,895 957,667

The protein

Protein family

• thiC family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-556 PubMed
• phosphorylated on Ser-565, Ser-586 and Tyr-589 PubMed

Cofactors

• Fe-S cluster PubMed

Structure

• 3EPN (from Caulobacter crescentus, 65% identity) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: thiC
  Description: PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, in Thi-box regulon

  Regulation

  • repressed by thiamine (Thi-box) PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• 1A603 ( thiC::erm), PubMed, available at BGSC
• BKE08790 (thiC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT, downstream forward: _UP4_TAAAAAAAATGAAGATGGAG
• BKK08790 (thiC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT, downstream forward: _UP4_TAAAAAAAATGAAGATGGAG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab

References

Reviews

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Original publications

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